Fig. 1.

Transcriptional activity of DuOx1 and −2 promoters in a rat thyroid cell line, PCCL3 (a), and in a nonthyroid cell line, HeLa (b). DuOx1 and −2 promoter transcription activity was measured in two different promoter constructs: DuOx1 (ptx43, 0.5 kpb, and ptx52, 6.1 kpb) and DuOx2 (ptx41, 0.6 kpb, and ptx46, 4.1 kpb). 3 µg of each DuOx promoter sequence cloned in pGL3 luciferase reporter vector coding for firefly luciferase was cotransfected with 0.02 µg of pRL renilla luciferase reporter vector to normalize luciferase activity in PCCL3 or HeLa cells. * p < 0.05 vs. ptx41. All transfections were done in triplicate, at least twice.