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. 2014 Dec 19;42(1):29–37. doi: 10.1159/000370168

Fig. 3.

Fig. 3

Influence of CCL15 on HPC adhesion strength. FDCP-mix A or freshly isolated Lin– bone marrow HPCs B, C were allowed to settle on immobilized VCAM-1 with or without chemokines for 3 min, before defined shear stress was applied, increasing stepwise every 30 s, as indicated. The percentages of remaining attached cells are given (100% correspond to initially settled cells). A Induction of adhesion in FDCP Mix HPCs or B in Lin– bone marrow cells by CXCL12, CCL15(27–92), or CCL15(1–92), (chemokine vs. control: + p < 0.05; ++ p < 0.01). C HPCs were pretreated with 200 μg/ml Bordetella Pertussis Toxin (Pertussis) or 100 μmol/l Rac inhibitor NSC 23766 for 2 h. Subsequently cells to immobilized to VCAM-1 plus CCL15(27–92) (CCL15(27–92) vs.CCL15(27–92) + Pertussis toxin + p < 0.05; ++ p < 0.01; CCL15(27–92) vs.CCL15(27–92) + NSC 23766: + p < 0.05; ++ p < 0.01). Results from 3 different experiments were pooled. In each experiment 4 microscopic fields were scored for each step of shear stress. Values are means ± SD.