Fig. 6.

Effect of CCL15 on hematopoietic repopulation in mice. Bone marrow cells from Ly5.1 mice (5 × 10⁶) were pre-treated with the indicated CCL15 isoforms for 30 min or not in IMDM/10% FCS, washed by centrifugation, admixed with a threefold excess of untreated Ly5.2 cells and transplanted into 8.5 Gy pre-irradiated Ly5.2 recipient mice in groups of 3–5 mice. Mice were sacrificed 3–4 weeks later, and bone marrow cells were analyzed by flow cytometry for expression of Ly5.1 and Ly5.2. Values are means ± SD from three experiments with a total of 8 (controls with untreated bone marrow cells), 9 (CCL15(1–92)-treated bone marrow cells) and 11 mice (CCL15(27–92)-treated bone marrow cells). *p < 0.05 versus control.