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. 2014 Dec 22;42(1):15–21. doi: 10.1159/000370217

Fig. 1.

Fig. 1

A The result of RNA electrophoresis. B The results of RT-PCR for the RHD-CE-D hybrid alleles. M: molecular marker; Lane 1: water control; Lane 2: amplicon of RHCE+ control with primer a and RHCE-specific reverse primer c (band of 1399 bp); Lane 3: amplicon of RHD+ control with primer a and RHD-specific reverse primer b (band of 1,446 bp); Lane 4–5: amplicons for RHD-CE(4–9)-D and RHD-CE(2–5)-D with primer a and RHD-specific reverse primer b (band of 1,446 bp); Lane 6–7: amplicons were lacking for RHD-CE(4–9)-D and RHD-CE(2–5)-D with primer a and RHCE-specific reverse primer c.