Table 1.
Primers used for PCR and DNA sequencing
| Primer name* | Sequence 5′ to 3′ | Specificity | Genomic region | Position§ | Product size (bp) |
|---|---|---|---|---|---|
| E1-s (=E1-seq) | TCCATAGAGAGGCCAGCACAA | D | promoter | −152 to −132 | |
| E1-a | GCTATTTGCTCCTGTGACCACTT | D | intron 1 | 40–18 | 340 |
| E2-s | TGACGAGTGAAACTCTATCTCGAT | D | intron 1 | −1,064 to −1,041 | |
| E2-a (=E2-seq) | GGCATGTCTATTTCTCTCTGTCTAAC | D/CE | intron 2 | 355–330 | 1,606 |
| E3-s | GTCGTCCTGGCTCTCCCTCTCT | D | intron 2 | −29 to −8 | |
| E3-a (=E3-seq) | CTTTTCTCCCAGGTCCCTCCT | D/CE | intron 3 | 39–19 | 219 |
| E4-s | GCCGACACTCACTGCTCTTAC | D/CE | intron 3 | −36 to −16 | |
| E4-a (=E4-seq) | TGAACCTGCTCTGTGAAGTGC | D | intron 4 | 194–174 | 378 |
| E5-s | CTGCCAAAGCCTCTACCCG | D | intron 4 | −502 to −484 | |
| E5-a (=E5-seq) | GCTGACTCTCGCTCATGGT | D/CE | intron 5 | 315–297 | 984 |
| E6-s (=E6-seq) | CAGGGTTGCCTTGTTCCCA | D/CE | intron 5 | −95 to −77 | |
| E6-a | CTTCAGCCAAAGCAGAGGAGG | D | intron 6 | 41–21 | 274 |
| E7-s (=E7-seq) | CTACTCATAGTGTGGTCCGTAGACC | D | intron 6 | −280 to −256 | |
| E7-a | CAAATATTCACCGAAGCCTACTG | D/CE | intron 7 | 129–107 | 543 |
| E8-s | GGTCAGGAGTTCGAGATCAC | D | intron 7 | −594 to −575 | |
| E8-a (=E8-seq) | GATGGGGCACATAGACATCC | D/CE | intron 8 | 97–78 | 771 |
| E9-s (=E9-seq) | GGTCCAGGAATGACAGGGCT | D | intron 8 | −162 to −143 | |
| E9-a | CGCTGAGGACTGCAGATAGG | D | intron 9 | 294–275 | 530 |
| E10-s (=E10-seq) | CAAGAGATCAAGCCAAAATCAGT | D/CE | intron 9 | −67 to −45 | |
| E10-a | AGCTTACTGGATGACCACCA | D | 3UTR | 290–271 | 382 |
| β-actin-s | GGAAATCGTGCGTGACATT | – | – | – | |
| β-actin-a | CGTCATACTCCTGCTTGCTG | – | – | – | 473 |
s = Sense primer; a = antisense primer; seq = sequencing primer.
§
The positions of the synthetic oligonucleotides are indicated relative to their distances from the first nucleotide position of the start codon ATG for all primers in the promoter and in the exons or relative to their adjacent exon-intron boundaries for all other primers.