Figure 6.

The Non-phosphorylatable SynIII Mutant at the CDK5 Phosphorylation Site Does Not Rescue shRNA-Induced Defects

(A) Confocal images of cortical coronal sections of animals at E21 transfected with shRNAscr (control), S404A rSynIII, S404A rSynIII/shRNA, or shRNA. The scale bar represents 100 μm.

(B) Quantification of the number of PNs residing in the VZ/SVZ, IZ, or CP in shRNAscr (control)-, S404A rSynIII-, S404A rSynIII/shRNA-, or shRNA-expressing cortices. The data are mean percentages (±SEM) of the total number of fluorescent cells in the section (one-way ANOVA; post hoc Holm-Sidak test: all conditions versus control ^∗∗∗p < 0.001).

(C) Confocal images of cortical coronal sections at P7 from animals transfected with shRNAscr (control), S404A rSynIII, S404A rSynIII/shRNA (0.5:1 ratio), S404A rSynIII/shRNA (1:1 ratio), or shRNA. The scale bars represent 100 μm. Arrowheads, example PNs that did not complete radial migration (black, UP neurons; gray, IN neurons).

(D) Quantification of the distribution of CP cells in the three orientation classes, as in Figure 1D (one-way ANOVA; post hoc Holm-Sidak test: ^∗p < 0.05; ^∗∗p < 0.01; ^∗∗∗p < 0.001). The data are mean percentages (±SEM) of the total number of CP cells.

(E) Quantification of the number of control, S404D rSynIII⁺, S404D rSynIII/shRNA⁺ (0.5:1 ratio), S404D rSynIII/shRNA⁺ (1:1 ratio), or shRNA⁺ cells that did not complete migration (one-way ANOVA; post hoc Holm-Sidak test: ^∗p < 0.05; ^∗∗p < 0.01; ^∗∗∗p < 0.001).

(F) Quantification of the percentage of inverted cells with respect to the total ectopic cells in the control, S404D rSynIII alone, S404D rSynIII/shRNA (0.5:1 ratio), and S404D rSynIII/shRNA (1:1 ratio) conditions (one-way ANOVA; post hoc Holm-Sidak test: ^∗p < 0.05; ^∗∗p < 0.01; ^∗∗∗p < 0.001).

The data in (E) and (F) are mean percentages (±SEM) of the total number of ectopic cells. See Figure S7.