Skip to main content
. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Brain Behav Immun. 2014 Dec 3;45:189–197. doi: 10.1016/j.bbi.2014.11.011

Figure 2. The enhancement of basal GABA release in the CeA of Il11rn KO mice is reversed by Kineret pretreatment.

Figure 2

A) Representative sIPSC recordings from CeA neurons from WT and Il1rn KO mice. Top panel: sIPSC traces from an untreated (left) and Kineret-pretreated neuron (right) from WT mice. Bottom panel: Left, the sIPSC frequency in an untreated neuron from a KO mouse is higher than in the WT mouse; Right, sIPSC trace from a Kineret pretreated neuron from an Il1rn KO mouse. B) The scatter graphs of sIPSC frequencies, amplitudes, and rise and decay times of individual CeA neurons from WT and Il1rn KO mice. The mean sIPSC frequency was higher (t32=2.36, p < 0.05)in KO (2.7 ± 0.5 Hz, n=21) compared to WT mice (1.0 ± 0.2 Hz, n=13), but there were no significant differences by genotype in the amplitude (WT: 77.2 ± 9.9 mV; KO: 69.6 ± 6 mV; t32=0.70, p > 0.05), rise time (WT: 1.6 ± 0.1 ms; KO: 1.8 ± 0.1 ms; t32=1.13, p > 0.05), or decay time (WT: 3.0 ± 0.5 ms; KO: 4.3 ± 0.8 ms; t32=1.16, p > 0.05). Kineret (100 ng/ml) pretreatment normalized the higher sIPSC frequency in KO and increased the rise and decay times of the basal spontaneous GABAA-mediated transmission in both strains. There were main effects of Kineret (F(1,54)=5.80, p < 0.05) and strain x Kineret interaction (F(1,54)=4.86, p < 0.05), with no main effect of strain on frequency. We also found main effects of Kineret (F(1,54) = 11.93, p < 0.01) and strain (F(1,54)=5.23, p < 0.05), without strain x Kineret interaction on the rise time in WT (untreated: 1.6 ± 0.1 ms vs. Kineret pretreated: 1.9 ± 0.1 ms) and KO mice (untreated: 1.8 ± 0.1 ms and Kineret pretreated: 2.3 ± 0.1 ms). Finally, there were main effects of Kineret (F(1,54)=34.24, p < 0.01) and strain (F(1,54)=5.31, p < 0.05), without interaction on the decay time (untreated WT: 3.0 ± 0.5 ms vs. Kineret pretreated WT: 7.1 ± 0.4 ms; untreated KO: 4.3 ± 0.8 ms vs. Kineret pretreated KO: 9.3 ± 0.9 ms).* indicates t-test comparison of WT vs. Il1rn KO; # indicates comparison of untreated to Kineret pretreated in Il1rn KO; ^ indicates main effect of Kineret pretreatment (two-way ANOVA followed by Bonferroni post hoc test).