Table 1.
Summary of the effects of ethanol and Kineret in CeA neurons from Il1rn WT and KO mice. Ethanol (44 mM) was applied by superfusion for 10–15 minutes. Kineret (100 ng/ml) was superfused for 30–90 minutes prior to recordings.
| Recordings | Parameters/Pharmacology | Baseline | Ethanol | Kineret Baseline | Kineret + Ethanol | |||
|---|---|---|---|---|---|---|---|---|
| KO vs. WT | WT | KO | WT | KO | WT | KO | ||
| eIPSPs | Amplitude | ↑ | ↑ | = | n.a. | n.a. | n.a. | n.a. |
| PPF | = | ↓ | = | n.a. | n.a. | n.a. | n.a. | |
| sIPSCs | Frequency | ↑ | ↑ | ↑# | = | = | ↑ | ↑ |
| Amplitude | = | = | = | = | = | = | = | |
| Rise time | = | = | = | ↑ | ↑ | = | = | |
| Decay time | = | ↑ | ↑ | ↑ | ↑ | = | = | |
| mIPSCs | Frequency | = | ↑ | ↑ | n.a. | n.a. | n.a. | n.a. |
| Amplitude | = | = | = | n.a. | n.a. | n.a. | n.a. | |
| Rise time | = | ↑ | ↑ | n.a. | n.a. | n.a. | n.a. | |
| Decay | = | = | = | n.a. | n.a. | n.a. | n.a. | |
| Tonic | GBZ |
KO: LS+/LTB− WT: LS−/LTB+ |
↑ | ↑ | n.a. | n.a. | n.a. | n.a. |
| THIP | = | n.a. | n.a. | n.a. | n.a. | n.a. | n.a. | |
Abbreviations: GBZ (gabazine; 100 μM), KO (Il1rn KO mice), LS (late spiking neuron), LTB (low-threshold bursting neuron), THIP (gaboxadol; 5 μM), WT (Il1rn WT mice).
Symbols: ↑ increase; # decrease in number of cells responding to ethanol compared to WT mice; = no change; ↓ decrease; n.a. not applicable; + presence of tonic GABA conductance; − lack of tonic GABA conductance.