Fig 5. Combinations of activators.
Synergy is defined as occurring where the double dose of either of the two individual activators are significantly less effective than the combination of single doses of both reagents. Reagents are labelled as with the suffix “a” indicating activator. Label without a number indicates the chosen (typically 50% activation) dose for the activator. The prefix “2” indicates a doubling of this dose. The prefix “90” indicates the dose chosen to approximate 90% activation by the reagent. “Log response" on the horizontal axis refers to ADP release, as measured by the log10 luminescence of the measured arbitrary absorbance units (AAU). Small *: significant difference from the indicated double dose activator, by one-tailed Wilcoxon test P < 0.05. Large *represents where both tests are significant (a and h). Combinations are shown for the following activator pairs (A) Ca and Aa (B) Ca and Ta (C) Ea and Aa (D) Ea and Ca (E) Ea and Ta (F) Ea and Xa (G) Ta and Aa (H) Xa and Aa (I) Xa and Ca (L) Xa and Ta.