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. Author manuscript; available in PMC: 2015 Apr 21.
Published in final edited form as: ACS Chem Biol. 2015 Jan 27;10(4):1054–1063. doi: 10.1021/cb5007536

Figure 2.

Figure 2

(A) Schematic of a fusion protein (HaloTag/R-GECO1.2) containing HaloTag (extracellular) and R-GECO1.2 (intracellular) linked by a transmembrane peptide. (B–E) Two-color imaging of cells transfected with HaloTag/R-GECO1.2 and labeled with fluorescent HaloTag substrates (1–2 μM) including HaloTag AF488 (B), Fluo HaloTag-1 (C), Fluo HaloTag-2 (D), or Fluo HaloTag-3 (E). The HaloTag substrates were cell impermeable so they only labeled HaloTag displayed on the cell surface. The fusion protein, however, was found to be localized at both the plasma and intracellular membranes after transient transfection. Scale bar = 5 μm.