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. 2015 Apr 21;11(4):e1005150. doi: 10.1371/journal.pgen.1005150

Fig 5. Both DDR and SAC components are required for efficient DNA damage repair and progeny viability after HU.

Fig 5

(A) Percent of nuclei that contain at least 1 RAD-51 focus—HU or 16 hours after 5mM HU recovery in wild-type, fzy-1(av15), mad-1(gk2), mad-2(RNAi), mad-3(ok1580), bub-3(RNAi), and atr(tm853) worms. Difference between—HU and +HU is statistically different in all genotypes except wild type and fzy-1(av15), p< 0.02 (two-way ANOVA)(n≥13). (B) Percent progeny inviability—HU and after 5mM HU exposure in WT and mad-3(ok1580); n≥13. *p<0.05, **p<0.001, ***p<0.0001 (two-way ANOVA). Error bars represent SEM. (C) Proliferative zone nucleus of WT worm stained for RAD-51 (white), NPC (red), MAD-2 (green) and DAPI (blue) in the presence of HU. Image represents one slice of a z-stack taken on SIM. Scale bar = 5μm.