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. 2015 Apr 21;10(4):e0124647. doi: 10.1371/journal.pone.0124647

Fig 3. p37 peptide suppresses cell growth and induces apoptosis in breast cell lines.

Fig 3

(A) Flow cytometric analysis of cell surface gp96 levels in breast cancer cells. (B) Cells were treated with 50 μg/ml of p37 for 48 h. Cellular apoptosis were analyzed by FACS, and the percentage of apoptotic cells (Annexin V single positive and Annexin V/PI double positive) was determined. (C) CCK-8 assay of cell proliferation upon treatment with various concentrations of p37 peptide for 48 h. Results are presented as means ± SD from three independent experiments. (D) SK-BR-3 cells were transfected with gp96 or control siRNA for 48 h. Cells were treated with 20 μg/ml of p37 or control peptide for additional 48 h. Cellular apoptosis were analyzed by FACS. Results are presented as means ± SD from three independent experiments. (E) Representative in vivo luciferase images of SK-BR-3 xenograft nude mice at day 21 after p37 (8 mg/kg) treatment. (F) T47D and Bcap-37 xenograft tumor volume in response to p37 (8 mg/kg) treatment. Data shown are the means and SDs of five mice. Data are representative of two independent experiments. **, P < 0.01 compared with controls.