Table 2.
Gene | Sequence | Annealing temp. | Cycles |
---|---|---|---|
β-actin | Forw. 5′-TTGGCAATGAGCGGTTCCGCTG-3′ Rev. 5′-TACACGTGTTTGCGGATGTCCAC-3′ |
55°C | 40x |
| |||
Vimentin, full length | Forw. 5′-GAGAACTTTGCCGTTGAAGC-3′ Rev. 5′-TCCAGCAGCTTCCTGTAGGTG-3′ |
55°C | 40x |
| |||
Vim3 | Forw. 5′-GAGAACTTTGCCGTTGAAGC-3′ Rev. 5′-GAAATAAAATGCTTACCCCTCAG-3′ |
55°C | 40x |