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. 2015 Apr 14;145(1):11–23. doi: 10.1111/imm.12393

Figure 3.

Figure 3

Regulatory T (Treg) cell stability in the periphery of Bcl6−/− mice. Data from the spleen of Bcl6+/+ (wild-type; WT) and Bcl6−/− (knockout; KO) Foxp3-GFP-Cre-Rosa-YFP (FCRY) mice, with representative flow plots on left and graphed data on right. Splenocytes were gated for either CD4+ (a) or CD4+ CD25+ (b) cells before GFP versus YFP analysis. The % of exTreg cells is calculated by the % GFP– YFP+ cells within the total YFP+ population. The numbers in large bold font in upper left and upper right quadrants are % exTreg cells and % stable Treg cells within the YFP+ population, and these percentages add up to 100%. The smaller numbers in italic font are the % of cells for each of the four quadrants and all four of these numbers add up to 100%. Graphs show average exTreg cells ± SE, with n = 8 to n = 12 mice of each genotype. Data from four separate experiments were combined into each graph. Each symbol represents one mouse. Student's t-tests were used to calculate statistical significance and P-values. NS, not significant (P > 0·05), *P < 0·05, ****P < 0·001.