Figure 2.

Infection with vΔN1 or vN1.I6E induces enhanced numbers of effector CD8⁺ T cells. Mice were infected intradermally with the indicated vaccinia viruses (VACVs) and populations of splenic GzmB^hi CD62L^lo CD8⁺ T cells were counted at 7, 14, 21 and 28 days post-infection. (a) Flow cytometry scatter plots from representative samples from individual mice. The arrows emphasize the greater percentage of GzmB^hi CD62L^lo CD8⁺ T cells following infection with vN1.I6E or vΔN1 compared with other viruses. (b) Graphs showing the proportion of total CD8⁺ T cells that were GzmB^hi CD62L^lo (left) and the absolute numbers of GzmB^hi CD62L^lo cells (right panel) (mean ± SEM). *P < 0·05, n = 5.