Figure 4.

Infection with vΔN1 or vN1.I6E induces enhanced numbers of effector CD8⁺ T cells in the vaccinia virus (VACV) -specific CD8⁺ T cells in the draining lymph nodes. Mice were infected intradermally with the indicated VACVs and populations of DimerX⁺ CD8⁺, and GzmB^hi CD62L^lo of DimerX⁺ CD8⁺ T cells in the draining lymph nodes were counted at 7 days post-infection. (a) Flow cytometry scatter plots from representative samples from individual mice. The arrows emphasize the greater percentage of DimerX⁺ CD8⁺ and GzmB^hi CD62L^lo of DimerX⁺ CD8⁺ T cells following infection with vN1.I6E or vΔN1 compared with other viruses. (b) Graphs showing the absolute numbers of DimerX⁺ CD8⁺ T cells (left) and GzmB^hi CD62L^lo of DimerX⁺ CD8⁺ T cells (right panel) (mean ± SEM). **P < 0·01, n = 5.