Figure 8.

Deletion or I6E mutation of N1 enhances vaccinia virus (VACV) -specific CD8⁺ central and effector memory T-cell (T_CM and T_EM) populations. Mice were infected intradermally with the indicated VACVs and populations of splenic lymphocytes that were DimerX⁺ CD8⁺, and T_EM or T_CM of DimerX⁺ CD8⁺ T cells, were counted at 28 days post-infection. (a) Flow cytometry scatter plots from representative samples from individual mice. The arrows emphasize the greater percentage of DimerX⁺ CD8⁺ following infection with vN1.I6E or vΔN1 compared with other viruses. (b) Graphs showing the absolute numbers of DimerX⁺ CD8⁺ T cells. (c) Graphs showing the absolute numbers of T_EM and T_CM of DimerX⁺ CD8⁺ T cells (mean ± SEM). *P < 0·05; n = 5.