Table 1.
Serum levels of IgM, IgG, IgG2b and autoantibodies
| Females | Males | |||
|---|---|---|---|---|
| Nba2 | Nba2.TLR8−/− | Nba2.Yaa | Nba2.TLR8−/Yaa | |
| IgM (μg/ml) | 699 ± 118 | 908 ± 65* | 1333 ± 497 | 4393 ± 1450** |
| IgM against dsDNA (U/ml) | 70 ± 9·5 | 70 ± 7·3 | 541 ± 240 | 1057 ± 413 |
| IgM against RNA (U/ml) | 55 ± 6·4 | 60 ± 6·4 | 7084 ± 2980 | 14 104 ± 4119 |
| IgG (mg/ml) | 13 ± 1·5 | 16 ± 0·6* | 11 ± 1 | 17 ± 1·6** |
| IgG2b (μg/ml) | 45·5 ± 12·9 | 69·3 ± 19·7 | 35·4 ± 16·6 | 121·7 ± 33·7** |
| IgG against dsDNA (U/ml) | 5·3 ± 0·7 | 6·5 ± 1·6 | 4·4 ± 1·6 | 15 ± 6·1 |
| IgG1 against dsDNA (U/ml) | 13·6 ± 4·1 | 34·9 ± 14·8 | 8·9 ± 1·7 | 43·7 ± 27·1 |
| IgG2b against dsDNA (U/ml) | 66·4 ± 14·9 | 57·8 ± 8·6 | 78·8 ± 13·5 | 259·8 ± 44·8*** |
| IgG2c against dsDNA (U/ml) | 3·9 ± 1·4 | 5·6 ± 1·8 | 15·8 ± 2·89 | 27·1 ± 3·7* |
| IgG3 against dsDNA (U/ml) | 0·9 ± 0·5 | 3·2 ± 1·2 | 0·9 ± 0·5 | 5·0 ± 2·7** |
| IgG against RNA (U/ml) | 107 ± 44 | 111 ± 26 | 504 ± 272 | 1087 ± 272 |
| IgG1 against RNA (U/ml) | 16·7 ± 3·8 | 70·1 ± 41·3 | 72·3 ± 41·1 | 227 ± 92 |
| IgG2b against RNA (U/ml) | 35·2 ± 8·0 | 228 ± 88 | 346 ± 114 | 881 ± 147** |
| IgG2c against RNA (U/ml) | 1·5 ± 1·1 | 8·9 ± 3·3 | 22·8 ± 7·5 | 36·0 ± 14·1 |
| IgG3 against RNA (U/ml) | 3·7 ± 1·2 | 4·2 ± 1·2 | 10·3 ± 4·5 | 252 ± 126* |
| IgG against Chromatin (U/ml) | 5·9 ± 1·1 | 9·4 ± 1·2 | 11 ± 1·2 | 12 ± 1·3 |
Serum was collected from 8-month-old Nba2 (n = 14), Nba2.TLR8−/− (n = 23), Nba2.Yaa (n = 9), and Nba2.TLR8−/Yaa (n = 12) mice. Levels were determined using ELISA. Values are the mean ± SEM.
*
P < 0·05;
**
P < 0·01
***
P < 0·001.