Table 2.
Subsets of circulating monocyte and splenic B cells, T cells and dendritic cells
| Cell type | Females | Males | ||
|---|---|---|---|---|
| Nba2 | Nba2.TLR8−/− | Nba2.Yaa | Nba2.TLR8−/Yaa | |
| Circulating monocytes | ||||
| Gr1+ | 5·5 ± 2·5 | 6·2 ± 1·7 | 8·6 ± 1·6 | 10·8 ± 1·8 |
| Gr1− | 4·3 ± 0·6 | 7·3 ± 3·2* | 11·9 ± 1·4 | 20·4 ± 2·8* |
| Total | 9·8 ± 3·0 | 13·5 ± 4·3* | 20·5 ± 2·9 | 31·1 ± 4·2* |
| Splenic populations | ||||
| T CD4+ | 22·7 ± 0·9 | 22·0 ± 1·0 | 16·3 ± 2·2 | 18·0 ± 0·4 |
| T CD8+ | 11·5 ± 0·6 | 8·9 ± 1·1 | 9·2 ± 1·7 | 10·9 ± 0·6 |
| B220+ | 51·7 ± 2·1 | 53·5 ± 2·6 | 43·0 ± 3·8 | 51·4 ± 2·1 |
| cDC | 1·7 ± 0·1 | 1·9 ± 0·1 | 2·4 ± 0·8 | 1·8 ± 0·1 |
| pDC | 0·29 ± 0·05 | 0·36 ± 0·05 | 0·21 ± 0·01 | 0·26 ± 0·03 |
Percentages of circulating monocyte subtypes (CD115+) were determined in 6-month-old Nba2 (n = 5), Nba2.TLR8−/− (n = 8) females, Nba2.Yaa (n = 14) and Nba2.TLR8−/Yaa (n = 12) male mice. Values are the mean ± SEM.
Percentages of splenic T cells (CD3+ gated), B cells and dendritic cells were determined in 4-month-old Nba2, Nba2.TLR8−/− females and Nba2.Yaa and Nba2.TLR8−/Yaa male mice (n = 3/group). Results are from one representative experiment. Three independent experiments were performed. Values are the mean ± SEM.
*
P < 0·05.
cDC, conventional dendritic cells; pDC, plasmacytoid dendritic cells.