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. 2015 Apr 14;145(1):161–169. doi: 10.1111/imm.12433

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© 2014 John Wiley & Sons Ltd

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Erk3 expression is induced during positive selection. Erk3^+/− thymocytes were stained with fluorescein digalactopyranoside (FDG) to detect Erk3 transcription. Erk3^+/+ thymocytes served as negative controls. (a) Histograms show FDG expression on total double-positive (DP) thymocytes, pre-selection DP thymocytes (CD69⁻) and post-selection DP thymocytes (CD69⁺). Representative of three independent experiments. (b) The expression of extracellular signal-regulated kinase (ERK3; FDG) is shown for DP1 (TCR^lo CD5^lo), DP2 (TCR^int CD5^hi) and DP3 (TCR^hi CD5^int) thymocytes. Bold numbers on histograms represent the FDG mean fluorescence intensity (MFI) of Erk3^+/− thymocytes while numbers in parenthesis represent the FDG MFI of Erk3^+/+ thymocytes.