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Phase contrast images confirming the effect of our purified mouse NGF on PC12 cultured for 6 consecutive days alone (A; untreated), with 50ng/ml NGF (B; NGF) and with 50ng/ml NGF pre-incubated with 500ng/ml neutralizing anti-NGF antibodies (C; ANA/NGF). Note the typical neuritis outgrowth from PC12 exposed to NGF (B), as compared to the absence of neuritis outgrowth in untreated (A) and ANA/NGF treated (C) PC12 cultures. (Magnification x200)
