Fig 7. Pclo1980-2553 is targeted into filopodia when co-expressed with activated Daam1.
(A) Labeling of mitochondria with Mitotracker Green (upper panels, green in merge) in COS7 cells expressing ActA-mRFP or ActA-Pclo1980-2553-mRFP (middle panels, red in merge) demonstrates localization of Pclo1980-2553 sequences to the surface of mitochondria. (B) Expression of ActA-mRFP (top set of panels, red in merge) or ActA-Pclo1980-2553-mRFP (bottom set of panels, red in merge) with Myc-C-Daam1 (green in merge) along with Actin labeling with Alexa fluor-coupled Phalloidin (blue in merge) reveals the accumulation of ActA-Pclo1980-2553-mRFP labeled mitochondria in phalloidin positive filopodia. (C) Analysis of mitochondrial expression pattern indicates nearly all cells expressing ActA-Pclo1980-2553-mRFP demonstrate accumulation in filopodia while this pattern is seen in less than half of the cells expressing ActA-mRFP. Data are expressed as mean with error bars representing standard deviation. For statistical analysis, comparison was made between the control cells (those expressing Mcy-C-Daam1 with ActA) and cells expressing Mcy-C-Daam1 with ActA coupled to Pclo1980-2553 using a two-tailed t-test (* p<0.05).