Fig 8. Immunization with emrA1-mAb complexes improves the extent of protection against Ft SchuS4 challenge.

(A) C57BL/6 mice (n = 10 per group) immunized i.n. with 1×10⁶ CFU of the emrA1 mutant-mAb immune complexes. On day 42 of the primary immunization mice were challenged i.n. with 32 CFU of Ft SchuS4. Age matched unvaccinated mice challenged with a similar dose of Ft SchuS4 served as controls. The survival results are expressed as Kaplan-Meier survival curves and P values were determined by Log-rank test. (B) The mice were weighed at the indicated times post-challenge to monitor the progression of infection. (C) The indicated Ft specific antibodies were determined in serum from immunized mice on day 14 post-immunization. The results are expressed as antibody titers.