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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Cell Immunol. 2015 Feb 14;295(1):19–28. doi: 10.1016/j.cellimm.2015.02.006

Fig. 2. Purification of Treg from PBMC of normal cynomolgus macaques.

Fig. 2

(A) Gating for flow sorting of Treg and Teff. Lymphocytes were gated based on forward (FSC) and side-scattered light (SSC), followed by single cell and CD4+ cell gating. Treg and Teff were gated on CD25+CD127−/low and CD25−CD127high, respectively, from CD4+ T lymphocytes. The percentages of putative Treg and Teff in CD4+ T cells are shown. (B) Representative flow cytometric plots of sorted Treg (left panel) and Teff (right panel). The numbers indicate the purity of Treg and Teff after sorting with a FACS Aria cell sorter. (C) Foxp3 expression by freshly-sorted putative Treg and Teff based on CD25 and CD127 gating. Histogram overlay from one representative experiment (left panel) and MFI from 3 separate experiments (right panel) are shown.