Skip to main content
. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Cell Immunol. 2015 Feb 14;295(1):19–28. doi: 10.1016/j.cellimm.2015.02.006

Fig. 6. Cryopreservation of expanded cynomolgus Treg does not affect their phenotype and suppressive capacity, but compromises their viability that can be restored by an additional round(s) of stimulation/expansion.

Fig. 6

Cynomolgus Treg were expanded for 1 or 2 rounds. At the end of each round, a portion of the cells was frozen. (A, B, C) Frozen expanded Treg were compared with their fresh counterparts in phenotype (A), tested as in Fig. 4, suppressive capacity (B), tested as in Fig. 5, and viability (C) tested by staining for Annexin V and PI. Data are representative of 3 experiments. (D) Cryopreserved expanded Treg were stimulated/expanded for an additional round (Frozen+st.) and compared to their fresh and frozen counterpart in viability. Data were graphed as % Annexin V+ cells, that include early apoptotic cells (Annexin V+ PI−) and late apoptotic cells (Annexin V+ PI+) cells. n>3. ****, p<0.0001;**, p<0.001; *, P<0.01.