Table 1. Dry weight (g), concentration of total Fe ([Fe]t), concentration of 57Fe in excess ([57Fe]e) and content of 57Fe in excess (57Fee) in the leaves, stems and roots measured in Citrus aurantium seedlings.
+Fe | -Fe | ANOVA | |||||
---|---|---|---|---|---|---|---|
Ct | S | Ct | S | Fe | S | Fe x S | |
DW (g) | |||||||
Leaves | 10.33a | 10.04a | 5.91b | 5.87b | *** | ns | ns |
Stems | 4.65a | 4.25b | 3.18c | 3.00c | *** | * | *** |
Roots | 2.86a | 2.07b | 2.50a | 1.81b | ** | *** | ns |
[Fe]t (μg Fe g-1 DW) | |||||||
Leaves | 75.85a | 35.87b | 31.21b | 25.61b | *** | *** | *** |
Stems | 6.42a | 4.20b | 4.90b | 4.25b | ** | *** | *** |
Roots | 496.72a | 248.08b | 127.42c | 114.83c | *** | *** | *** |
[57Fe]e (μg 57Fe g-1 DW) | |||||||
Leaves | 0.94b | 0.68c | 2.49a | 0.70c | *** | *** | *** |
Stems | 0.29b | 0.20c | 0.70a | 0.17c | *** | *** | *** |
Roots | 3.59b | 2.52c | 7.33a | 2.48c | *** | *** | *** |
57Fee (μg 57Fe plant-1) | |||||||
Whole seedling | 21.35b | 12.89c | 35.16a | 9.13c | *** | *** | *** |
Aerial part | 11.06b | 7.68c | 16.81a | 4.65c | *** | *** | *** |
Roots | 10.29b | 5.21c | 18.35a | 4.48c | *** | *** | *** |
Plants were grown for 21 days in Fe-sufficient (+Fe) or Fe-deficient (-Fe) nutrient solutions with the non-stressed (Ct) or the flooding treatment (S). For 57Fe determinations, plants were labelled immediately after treatments with 100 μM o,o-57FeEDDHA for 24 h.
1Values for DW and [Fet] are the means of six independent plants per treatment (n = 6). Values for [57Fee] and 57Fee are the means of three independent experiments (n = 3). For a comparison of the means, an ANOVA followed by the Fisher´s least significant differences (LSD) test, calculated at the 95% confidence level, was performed. Different letters in the same row indicate significant differences between the different genotypes and treatments. Levels of significance are represented by P < 0.05 (*), P < 0.01 (**), P < 0.001 (***) and ns (non-significant).