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. 2015 Apr 21;10(4):e0123644. doi: 10.1371/journal.pone.0123644

Table 1. Dry weight (g), concentration of total Fe ([Fe]t), concentration of 57Fe in excess ([57Fe]e) and content of 57Fe in excess (57Fee) in the leaves, stems and roots measured in Citrus aurantium seedlings.

+Fe -Fe ANOVA
Ct S Ct S Fe S Fe x S
DW (g)
 Leaves 10.33a 10.04a 5.91b 5.87b *** ns ns
 Stems 4.65a 4.25b 3.18c 3.00c *** * ***
 Roots 2.86a 2.07b 2.50a 1.81b ** *** ns
[Fe]t (μg Fe g-1 DW)
 Leaves 75.85a 35.87b 31.21b 25.61b *** *** ***
 Stems 6.42a 4.20b 4.90b 4.25b ** *** ***
 Roots 496.72a 248.08b 127.42c 114.83c *** *** ***
[57Fe]e (μg 57Fe g-1 DW)
 Leaves 0.94b 0.68c 2.49a 0.70c *** *** ***
 Stems 0.29b 0.20c 0.70a 0.17c *** *** ***
 Roots 3.59b 2.52c 7.33a 2.48c *** *** ***
57Fee (μg 57Fe plant-1)
 Whole seedling 21.35b 12.89c 35.16a 9.13c *** *** ***
 Aerial part 11.06b 7.68c 16.81a 4.65c *** *** ***
 Roots 10.29b 5.21c 18.35a 4.48c *** *** ***

Plants were grown for 21 days in Fe-sufficient (+Fe) or Fe-deficient (-Fe) nutrient solutions with the non-stressed (Ct) or the flooding treatment (S). For 57Fe determinations, plants were labelled immediately after treatments with 100 μM o,o-57FeEDDHA for 24 h.

1Values for DW and [Fet] are the means of six independent plants per treatment (n = 6). Values for [57Fee] and 57Fee are the means of three independent experiments (n = 3). For a comparison of the means, an ANOVA followed by the Fisher´s least significant differences (LSD) test, calculated at the 95% confidence level, was performed. Different letters in the same row indicate significant differences between the different genotypes and treatments. Levels of significance are represented by P < 0.05 (*), P < 0.01 (**), P < 0.001 (***) and ns (non-significant).