Fig. 1.

Expression of iron transport genes in Caco-2 cells over time following cell seeding in Transwell^® permeable support plates. Values shown indicate relative mRNA levels for a TfR-1, b DMT-1 and c FPN in Caco-2 cells determined at different days ranging from 10 to 21 post-seeding cultured with normal medium. Bars and error bars indicate mean + SD (n = 4 comprised of samples generated from 1 well from each of two separate plates set up in parallel in two independent experiments). Data were compared by one-way ANOVA with Tukey’s post hoc test. Asterisks indicate statistically significant differences between days (*p < 0.05, **p < 0.01 and ***p < 0.001)