Table 1.

Real-time RT-qPCR primer and probe sequences, amplicon lengths and mRNA variants amplified

Gene
POLR2A
Forward primer	5′ GCAAATTCACCAAGAGAGACG 3′
Reverse primer	5′ CACGTCGACAGGAACATCAG 3′
Probe	5′ [FAM]CCTGGAGC[TAMRA] 3′
Amplicon length	73 nucleotides
mRNA variants amplified	NM_000937.4
DMT-1 (SLC11A2)
Forward primer	5′ GATGTAGAGCATCTAACAGGGATGAA 3′
Reverse primer	5′ CCATTGGCAAAGTCACTCATTACT 3′
Probe	5′ [FAM]TCTACAGAGCTTACAGCTTCCCTTTGCTCT[TAMRA] 3′
Amplicon length	128 nucleotides
mRNA variants amplified	NM_001174125.1, NM_001174126.1, NM_001174127.1, NM_000617.2, NM_001174128.1, NM_001174129.1, NM_001174130.1
FPN (SLC40A1)
Forward primer	5′ GCCCATAATCTCTGTCAGTCTGC 3′
Reverse primer	5′ TGCCTCTTTCAGATTCAATTACATTT 3′
Probe	5′ [FAM]AATCAAAGGACCAAAGACCGATTCTAGCA[TAMRA] 3′
Amplicon length	122 nucleotides
mRNA variants amplified	XM_005246504.1, XM_005246505.1
TfR-1 (TFRC)
Forward primer	5′ GCTTTCCCTTTCCTTGCATATTCT 3′
Reverse primer	5′ GGTGGTACCCAAATAAGGATAATCTG 3′
Probe	5′ [FAM]ATCCCAGCAGTTTCTTTCTGTTTTTGCGA[TAMRA] 3′
Amplicon length	87 nucleotides
mRNA variants amplified	NM_001128148.1, NM_003234.2