Table 3.
Neural retinal and/or RPE genes expressed within human embryo stem cells induced by cultured on PA6 cells
| Probe set ID | Gene title | Gene symbol | GO biological process description | Present in |
|---|---|---|---|---|
| 204534_at | Vitronectin | VTN | Cell adhesion; cell-matrix adhesion | Retina |
| 206825_at | Oxytocin receptor | OXTR | GPCRDB Class A Rhodopsin-like; phospholipase C activating | Both |
| 209420_s_at | Sphingomyelin phosphodiesterase 1 | SMPD1 | signal transduction; nervous system development | Both |
| 211896_s_at | Decorin | DCN | Organ morphogenesis; integral to membrane | Retina |
| 212494_at | Tensin-like C1 domain containing phosphatase (tensin 2) | TENC1 | Intracellular singing cascade; Zinc ion binding; diacylglycerol binding | Retina |
| 212956_at | TBC1 domain family, member 9 (with GRAM domain) | TBC1D9 | Regulation of Rab GTPase activity | Both |
| 213540_at | Hydroxysteroid (17-beta) dehydrogenase 8 | HSD17B8 | Steroid biosynthetic process; lipid biosynthetic process | Both |
| 217522_at | Potassium channel, subfamily V, member 2 | KCNV2 | Ion transport; visual perception; response to stimulae | Retina |
| 218501_at | Rho guanine nucleotide exchange factor (GEF) 3 | ARHGEF3 | Rho protein signal transduction | Both |
| 227178_at | CUG triplet repeat, RNA binding protein 2 | CUGBP2 | RNA processing; neuromuscular junction development | Both |
| 228268_at | Flavin containing monooxygenase 2 (non-functional) | FMO2 | Oxygen and reactive oxygen species metabolic process | Retina |
| 230242_at | Neurofascin homolog (chicken) | NFASC | Cell adhesion; proteinaceous extracellular matrix | Both |
| 230475_at | Similar to RIKEN cDNA 6030419C18 gene | LOC388135 | Unknown | Retina |
| 233333_X_at | Advillin | AVIL | Cytoskeleton organization and biogenesis; nervous system development | Both |
| 243504_at | Transcribed locus | – | Unknown | Retina |
| 1555968_a_at | CDNA FLJ30424, similar to ZINC FINGER PROTEIN 195 | – | Unknown | Both |
| 1556281_at | Full length insert cDNA clone YI54A07 | YI54A07 | Unknown | Both |
| 1556464_a_at | Hypothetical protein LOC257407 | LOC257407 | Unknown | Retina |
| 1558401_at | CDNA FLJ37332 fis, clone BRAMY2019710 | – | Unknown | Retina |
| 1560792_at | CDNA FLJ37191 fis, clone BRALZ2003453 | FLJ37191 | Unknown | RPE |
| 1569078_at | Ring finger protein 213 | RNF213 | Nucleotide, protein AND zinc ion binding: nucleoside-triphosphatase activity | Retina |
| 1570155_at | Homo sapiens, clone IMAGE:4427279, mRNA | – | Unknown | RPE |
Culturing hESC on PA6 cells induced the expression of 22 new genes that were also expressed in neural retina and RPE. The functions of these 22 genes were diverse but were related to cell proliferation, nervous system development and cell adhesion.