Figure 1.
Bexarotene increases microglial phagocytosis in vitro. N9 microglia were incubated with 1 μm polystyrene beads, and their uptake was measured with flow cytometry (A). N9 cells were treated with or without nuclear receptor agonists (50 nm Bex, RXR agonist; 100 nm pio, PPARγ agonist; 100 nm GW3965, LXR agonist; GW0742, PPARδ agonist) for 18 h before incubation with 1 μm polystyrene beads, and the fold change in phagocytic index was measured (B). fAβ (2.5 μm) was added 30 min before bead uptake (C). Gas6 (40 nm) was added 30 min before bead uptake (D). Data represent three separate experiments; 10,000 cells were analyzed via flow cytometry. *p < 0.05. **p < 0.01. ***p < 0.001.