| Prothrombin time (PT) |
Performed according to the technique by Quick using human placental thromboplastin (Thromborel S, Siemens Healthcare Diagnostics Products GmbH, Marburg, Germany) |
Plasma/ 2 hours |
Automated coagulation analyzer BCS XP System (Siemens, Marburg, Germany) |
ratio |
| Activated partial thromboplastin time (APTT) |
Performed by using Actin FS (Dade Actin FS Activated PTT Reagent, Siemens Healthcare Diagnostics Products GmbH, Marburg, Germany) containing the purified soy phosphatides |
Plasma/ 2 hours |
Automated coagulation analyzer BCS XP System (Siemens, Marburg, Germany) |
ratio |
| Thrombin time (TT) |
Performed according to the instructions from manufacturer using bovine thrombin (BC Thrombin Reagent, Siemens Healthcare Diagnostics Products GmbH, Marburg, Germany) |
Plasma/ 2 hours |
Automated coagulation analyzer BCS XP System (Siemens, Marburg, Germany) |
sec |
| Fibrinogen concentration (FIB) |
Determinated by a modification of Clauss' coagulometric method using bovine thrombin (Multifibren U, Siemens Healthcare Diagnostics Products GmbH, Marburg, Germany) |
Plasma/ 2 hours |
Automated coagulation analyzer BCS XP System (Siemens, Marburg, Germany) |
g/L |
| Platelet count (PLT) |
Performed by using Coulter principle (impedance) (Beckman Coulter Reagents) |
Whole blood/ 2 hours |
Automated Coulter HmX Hematology Analyzer (Beckman Coulter Inc., Florida, USA) |
109/L |
| Hematocrit (HTC) |
Performed by directly measuring from RBC histogram |
Whole blood/ 2 hours |
Automated Coulter HmX Hematology Analyzer (Beckman Coulter Inc., Florida, USA) |
L/L |
