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. 2015 Apr 20;4:10.3402/jev.v4.26316. doi: 10.3402/jev.v4.26316

Fig. 1.

Fig. 1

DiR labelling does not affect EV morphology and co-localizes with EVs. (A) Western Blot (WB) stained for Alix and Tsg101 on HEK293T purified, DiR-labelled EVs and cell lysate (CL) (whole WB-membrane shown in Supplementary Fig. 1A). (B) NTA profile of DiR-labelled HEK293T EVs showing an EV-like distribution with a mode size around 100 nm. The curve has been normalized to dilution factors. (C) Electron microscopy picture of DiR-labelled HEK-293T EVs. Scale bar represents 100 nm. (D) Image depicting DiR-EVs and free DiR sucrose gradients in UC tubes and subsequent 12-well plates containing the different fractions. (E) Fluorescent values (normalized to background) of the DiR-EVs and free DiR gradients (assayed from the 12-well plates depicted in 1D). (F) WB stained for Alix for DiR-EVs from fractions 2–10 (whole WB-membrane shown in Supplementary Fig. 1B).