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. 2015 Apr 21;35(10):1741–1753. doi: 10.1128/MCB.01475-14

FIG 5.

FIG 5

PATZ1 inhibits p53 function. PATZ1 inhibits p53 activity in luciferase assays. (Top) HCT116 p53−/− cells transfected with different combinations of the pG13 p53-dependent luciferase vector and FLAG-p53, PATZ1, and PATZ1Alt expression plasmids. p53 specifically activates the pG13 reporter (columns 1 and 2), while PATZ1 and PATZ1Alt cannot (columns 3 and 4). PATZ1 but not PATZ1Alt can inhibit p53 activity (columns 5 and 6). (Middle and bottom) The same experiment was conducted with the p21 promoter luciferase construct (middle) and the Puma promoter luciferase construct (bottom).