FIG 2.

LPS upregulates RNA polymerase III activity. Mouse RAW264 macrophages (A), murine primary bone marrow-derived macrophages (BMDM) (B), and human THP-1 cells differentiated into macrophages (dTHP-1) (C) were treated with 1 μg/ml LPS and harvested at the indicated time points. RNA was isolated from cells and transcript levels of the indicated genes were assessed using RT-qPCR. RNA levels were normalized to GAPDH (A and B) or RPLP0 mRNA (C), and the value for the zero time point or the NT (nontreated) cells was set to 1. An asterisk indicates a P value of <0.05. Error bars represent SEM. The experiments were performed three times each in duplicate (A), five times (B), and four times (C).