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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: Biomaterials. 2015 Mar 24;53:621–633. doi: 10.1016/j.biomaterials.2015.02.121

Figure 5.

Figure 5

The effect of shear stress (24 hours) on the gene expression, relative to static, of the mechanosensory complex comprised of (A) CD31, (B) VE-cadherin and (C) KDR in HUVECs, HAECs and hiPSC-ECs (left to right) as shown by qRT-PCR. Both low shear (red bar, 5 dyne/cm2) and high (green bar, 10 dyne/cm2) are normalized to static conditions (blue bar). All conditions are also normalized to internal house-keeping gene GAPDH. (D) Phase-contrast images of morphology of hiPSC-ECs on porous membrane in static conditions (left), alignment under shear (right, arrow pointing in direction of flow) and phalloidin showing actin stress fibers (*p < 0.05, n=3 biological replicates, n=3 technical replicates each, Student’s t-test for samples within groups).