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. 2015 Feb 27;593(Pt 8):2071–2084. doi: 10.1113/jphysiol.2014.287060

Figure 5. Effect of clenburerol on EDL myofibrilar ATPase activity in Ca2+-activated conditions at 4°C.

Figure 5

Reaction mixture concentrations were 3 and 30 μm for myosin heads and [γ-32P]ATP concentrations in Rapid Flow Quench experiments, respectively. CTL-G21, control group; CB-G9, 9-day clenbuterol-treated group; CB-G14, 14-day clenburerol-treated group; CB-G21, 21-day clenburerol-treated group; k4, rate constant of Pi release (the isomerization step preceding diffusion of Pi); kF, rate constant of the fast steady-state phase in activated condition; tB, duration of the unloaded shortening phase, an index of unloaded shortening velocity; ATPtB, ATP consumed during the unloaded shortening phase. Because no significant difference was detected between CTL-G9, CTL-G14 and CTL-G21 groups, results of the CTL-G21 group are presented. NS, not significant.