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. Author manuscript; available in PMC: 2015 Apr 22.
Published in final edited form as: Circ Res. 2013 Nov 21;114(3):444–453. doi: 10.1161/CIRCRESAHA.114.302670

Figure 7. Defective extracellular signal–regulated protein kinase 1/2 (ERK1/2) signaling in Grb2−/− and Lat−/− platelets.

Figure 7

Determination of mitogen-activated protein kinase (MAPK) phosphorylation. Washed platelets (5×108/mL) from Grb2+/+ and Grb2−/− mice (A) or Lat+/+ and Lat−/− mice (B) were stimulated with 0.5 μg/mL convulxin (CVX) under stirring conditions at 37°C. Aliquots were taken at the indicated time points and subsequently lysed with NP-40 detergent. Proteins were separated by reducing SDS-PAGE (10%), blotted on a PVDF membrane, and stained using the indicated phospho-specific antibodies. Staining of the respective nonphosphorylated proteins, GAPDH or tubulin served as loading controls. The result shown is representative of 3 individual experiments.