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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: J Mol Cell Cardiol. 2015 Feb 11;82:194–200. doi: 10.1016/j.yjmcc.2015.01.014

Figure 6. HIF-1α expression in the heart attenuates accumulation of adenosine in ischemic hearts where glycolytic activity has been blocked.

Figure 6

Hearts were obtained from WT mice and those in which the HIF-1α-PPN transgene was expressed for 6 days (6D). A 25 minute pre-ischemic perfusion period was performed in order to allow stabilization of the hearts. Then, they were perfused with glucose-free Krebs buffer containing 5 mM glycolytic inhibitor iodoacetate for 2.5 minutes prior to 20 minutes of total ischemia. After ischemic challenge, nucleotides were extracted from the hearts and their contents were measured using HPLC. * P<0.05 versus WT. n=4-5.