Table 1.
Summary of the distribution of adenine nucleotides and their breakdown products in wildtype mouse hearts as well as those in which the HIF-1α-PPN transgene has been expressed for 6 days (6D). Nucleotide levels were measured in mouse hearts prior to ischemia, after 30 minutes of ischemia or after perfusion with 50 μg/ml PNC inhibitor hadacidin followed by 30 minutes of ischemia.
| WT 30 minutes of ischemia |
6D 30 minutes of ischemia | |||||
|---|---|---|---|---|---|---|
| WT pre- ischemic |
(−) hadacidin |
(+) hadacidin |
6D pre- ischemic |
(−) hadacidin |
(+) hadacidin |
|
| ATP | 26.44±3.71 | 2.00±0.22‡ | 0.32±0.03‡§ | 21.44±2.37 | 4.44±0.55*‡ | 0.73±0.07*‡§ |
| ADP | 8.92±1.19 | 2.72±0.21‡ | 1.79±0.12‡§ | 8.84±1.32 | 6.04±0.29* | 3.23±0.29*‡§ |
| AMP | 2.76±0.57 | 5.13±0.78‡ | 8.77±0.59‡§ | 3.33±0.99 | 8.42±0.94‡ | 14.88±2.06*‡§ |
|
Energy
charge |
0.79±0.04 | 0.35±0.04‡ | 0.11±0.01‡§ | 0.77±0.05 | 0.40±0.02‡ | 0.13±0.01‡§ |
| ATP:ADP | 3.29±0.60 | 0.76±0.10‡ | 0.18±0.01‡§ | 3.05±0.63 | 0.73±0.07‡ | 0.23±0.02‡§ |
|
total adenine
nucleotide |
38.12±3.91 | 9.85±0.71‡ | 10.89±0.64‡ | 33.61±2.57 | 18.91±1.54*‡ | 18.83±2.33*‡ |
| adenosine | 0.41±0.08 | 3.19±0.92‡ | 6.82±0.77‡§ | 0.50±0.14 | 1.04±0.21*‡ | 1.00±0.20* |
| IMP | 0.94±0.29 | 3.13±0.65‡ | 3.81±0.28‡ | 0.68±0.18 | 1.26±0.29* | 1.38±0.11*‡ |
| inosine | 0.14±0.06 | 4.43±0.55‡ | 5.2±0.24‡ | 0.25±0.07 | 3.26±0.46‡ | 3.08±0.26*‡ |
| hypoxanthine | 0.14±0.06 | 1.42±0.07‡ | 1.47±0.06‡ | 0.19±0.05 | 1.38±0.09‡ | 1.29±0.05‡ |
‡
P<0.05 versus the pre-ischemic value for the corresponding group.
§
P<0.05 versus hearts not treated with hadacidin.
*
P<0.05 versus wildtype. n=3-9.