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. 2015 Apr 11;14:51. doi: 10.1186/s12934-015-0236-2

Table 2.

Comparison of production of 3HB by E. coli AF1000 and PPA652ara by varied cultivation techniques

Process mode Sugar Strain Time CDW Sugar consumed 3HB HAc mean q S total mean q 3HB Y 3HB/HAc Y 3HB/CDW Y 3HB/Sugar
(h) (g L −1 ) (g L −1 ) (g L −1 ) (g L −1 ) (g g −1h −1 ) (mg g −1h −1 ) (g g −1 ) (g g −1 ) (g g −1 )
Batch Glc AF1000 5.5 4.18 8.90 0.51 0.96 1.0 68 0.54 0.12 0.06
Batch Glc + Xyl + Ara PPA652ara 7.3 4.88 9.65 0.31 0.00 0.7 25 → ∞ 0.06 0.03
N-depletion Glc + Xyl + Ara AF1000 16.0 0.27 2.73 0.64 0.48 0.2 29 1.33 2.37 0.23
N-depletion Glc + Xyl + Ara PPA652ara 15.0 0.39 3.79 0.54 0.54 0.3 31 1.00 1.38 0.14
N-fedbatch Glc + Xyl + Ara AF1000 11.0 10.37 37.05 1.84 2.66 0.6 45 0.69 0.18 0.05
N-fedbatch Glc + Xyl + Ara PPA652ara 11.0 11.82 36.75 1.87 0.77 0.6 38 2.43 0.16 0.05

In the cases of nitrogen depletion and nitrogen-limited fed-batch, the values given in the table are calculated for the respective depleted/limited phase only, and do not include the batch phases.