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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Circ Heart Fail. 2015 Feb 10;8(2):362–372. doi: 10.1161/CIRCHEARTFAILURE.114.001274

Figure 6. Age-related increase in canonical Wnt signaling in sFRP-KO hearts.

Figure 6

(A-B) Protein expression of β-catenin analyzed by western blotting in control sFRP-1 WT hearts versus sFRP-1 KO hearts at 6 (A) and 12 (B) months of age. ß-Actin was used as a housekeeping loading control. (C) Representative immunofluorescent staining for β-Catenin merged with DAPI in the myocardium of sFRP-1 KO compared with WT hearts at 6 and 12 months of age. Magnification is 20×. Scale bars represent length of 100μm. (D-E) mRNA analysis of β-Catenin and Lef1 in sFRP-1 KO vs WT hearts at 6 and 12 months of age (n=5). ß-actin was used as a housekeeping control, data is presented as fold of regulation. Significant differences were denoted by *P < 0.05 < or **P < 0.01, or ***P < 0.001. > vs control littermate hearts. All values were expressed as mean ± SEM.