Fig. 1.

2D gel profiles of whole cell lysate proteins of Mtb clinical isolates collected sequentially from a single patient. The upregulated proteins are highlighted by circles. (A) First Mtb isolate (before treatment) sensitive to all 4 drugs, (B) Second isolate (during treatment) acquires MDR, (C) Third isolate (after 15 months of treatment) acquires drug resistance to yet another drug kanamycin and (D) Fourth MDR isolate after 27 months. Two Proteins, A (Rv1080c) and B (Rv2140c) marked in green rectangles, were selected for observing expression variation in all four samples and showed similar level of expression in all gels.