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. 2015 Jan;141(1):27–45. doi: 10.4103/0971-5916.154492

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Copyright: © Indian Journal of Medical Research

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 4 — Detection of mRNA expression in drug treated Mtb isolates. Isolates treated with isoniazid (INH, 0.1μg/ml), rifampicin (RIF, 1.0μg/ml), ethambutol (EMB, 5μg/ml) and INH (0.1μg/ml) +RIF (1.0μg/ml). The expression is represented in fold increase. The 16sRNA was used as internal control for mRNA expression analysis. mRNA expression of (A) ahpC, (B) Rv1827, (C) pknA, (D) pknB, (E) pknG and (F) wag31. Calculation and statistical analysis carried out by using the 2^-∆∆CT method and results presented in fold increase.