Figure 2.

A. thaliana seedlings (11 d) exposed to 0.01–0.1 M AITC for 1–6 h displayed a dose- and time-dependent depletion of GSH-pool. (A) An effect which proved to be reversible when AITC-treated seedlings (0.01 M) were allowed to recover in an AITC-free environment (B). Values represent means ± s.d. of 3 replicates. The stapled line depicts the time when AITC-treated seedlings were transferred for recovery. A. thaliana (11 d) also displayed loss of chlorophyll 1–5 days following 1 h 0.5–3 M AITC-treatments (C). Values represent means ± s.d. of 4 replicates. (D) Staining of ROS in roots with H₂DCFDA following treatment with vapor of AITC showed an increased intensity when observed under a fluorescene microscope. Statistical analysis was performed with a two-tailed t-test, ^*p < 0.05.