Table 2.
Yeast species isolated from dried crimped barley (D); moist crimped barley (M); moist crimped barley inoculated with Wickerhamomyces anomalus (W); moist crimped barley inoculated with Wickerhamomyces anomalus and LAB starter culture (WLAB).
| Treatment | D | M | W | WLAB | ||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Week | 7 | 9 | 11 | 13 | 15 | 17 | 19 | 7 | 9 | 11 | 13 | 15 | 17 | 19 | 7 | 9 | 11 | 13 | 15 | 17 | 19 | 7 | 9 | 11 | 13 | 15 | 17 | 19 |
| Acremonium strictum | 2 | b.d.1 | b.d.1 | b.d.1 | ||||||||||||||||||||||||
| Aureobasidium pullulans | 3 | 1 | b.d.1 | b.d.1 | b.d.1 | |||||||||||||||||||||||
| Debaryomyces hansenii | 3 | 1 | 1 | 5 | 3 | b.d.1 | b.d.1 | b.d.1 | ||||||||||||||||||||
| Pichia fermentans | 3 | b.d.1 | b.d.1 | b.d.1 | ||||||||||||||||||||||||
| Pichia kudriavzevii | 2 | 5 | 4 | 5 | 8 | 8 | 7 | b.d.1 | 10 | 10 | 8 | 7 | 2 | b.d.1 | 10 | b.d.1 | ||||||||||||
| Saccharomyces castellii | b.d.1 | 2 | b.d.1 | b.d.1 | ||||||||||||||||||||||||
| Saccharomyces cerevisiae | 3 | b.d.1 | b.d.1 | b.d.1 | ||||||||||||||||||||||||
| Wickerhamomyces anomalus | 3 | 2 | 5 | 6 | 5 | 2 | 2 | 3 | b.d.1 | 10 | 10 | 10 | 10 | 2 | 3 | 8 | 8 | 10 | b.d.1 | b.d.1 | ||||||||
| Cryptococcus magnus | 1 | b.d.1 | b.d.1 | b.d.1 | ||||||||||||||||||||||||
| Cryptococcus victoria | 1 | 1 | 4 | b.d.1 | b.d.1 | b.d.1 | ||||||||||||||||||||||
| Rhodotorula graminis | 7 | 2 | 3 | 2 | 1 | b.d.1 | b.d.1 | b.d.1 | ||||||||||||||||||||
| Sporobolomyces ruberrimus | 4 | 7 | 3 | 2 | b.d.1 | b.d.1 | b.d.1 | |||||||||||||||||||||
Samples were taken after 7 weeks of storage and then consecutively every 2 weeks until week 19. Numbers in columns reflect the species frequency among 10 identified isolates per sampling occasion. Species were identified by D1D2 rDNA gene sequencing using primers NL1/NL4.
1
Below detection level (100 cfu g−1 grain).