Table 3.
Lactic acid bacteria (LAB) species isolated from dried crimped barley (D); moist crimped barley (M); moist crimped barley inoculated with Wickerhamomyces anomalus (W); moist crimped barley inoculated with Wickerhamomyces anomalus and LAB starter culture (WLAB).
| Treatment | D | M | W | WLAB | ||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Week | 7 | 9 | 11 | 13 | 15 | 17 | 19 | 7 | 9 | 11 | 13 | 15 | 17 | 19 | 7 | 9 | 11 | 13 | 15 | 17 | 19 | 7 | 9 | 11 | 13 | 15 | 17 | 19 |
| Lactobacillus curvatus | b.d.1 | b.d.1 | b.d.1 | b.d.1 | 2 | |||||||||||||||||||||||
| Lactobacillus brevis | b.d. | b.d. | b.d. | b.d. | 2 | 1 | 4 | 1 | 1 | 6 | 1 | |||||||||||||||||
| Lactobacillus buchneri | b.d. | b.d. | b.d. | b.d. | 2 | 1 | ||||||||||||||||||||||
| Lactobacillus coryniformis | b.d. | b.d.1 | b.d.1 | b.d.1 | 1 | 1 | 4 | 1 | 2 | 2 | ||||||||||||||||||
| Lactobacillus crustorum | b.d.1 | b.d.1 | b.d.1 | b.d.1 | 2 | |||||||||||||||||||||||
| Lactobacillus paralimentarius | b.d.1 | b.d.1 | b.d.1 | b.d.1 | 1 | 1 | ||||||||||||||||||||||
| Lactobacillus plantarum | 5 | 6 | 4 | b.d.1 | b.d.1 | b.d.1 | b.d.1 | 4 | 1 | 10 | 4 | 6 | 3 | 1 | 6 | 9 | 9 | 9 | 6 | 2 | 10 | 10 | 10 | 7 | 10 | 7 | 9 | 8 |
| Leuconostoc citreum | 2 | 1 | 4 | b.d.1 | b.d.1 | b.d.1 | b.d.1 | |||||||||||||||||||||
| Leuconostoc mesenteroides | 2 | b.d.1 | b.d.1 | b.d.1 | b.d.1 | |||||||||||||||||||||||
| Pediococcus parvulus | b.d.1 | b.d.1 | b.d.1 | b.d.1 | 1 | |||||||||||||||||||||||
| Pediococcus cellicola | b.d.1 | b.d.1 | b.d.1 | b.d.1 | 1 | 1 | ||||||||||||||||||||||
| Pediococcus pentosaceus | 3 | 3 | b.d.1 | b.d.1 | b.d.1 | b.d.1 | 4 | 9 | 6 | 2 | 2 | 2 | 4 | 1 | ||||||||||||||
| Weissella paramesenteroides | b.d.1 | b.d.1 | b.d.1 | b.d.1 | ||||||||||||||||||||||||
Samples were taken after 7 weeks of storage and then consecutively every 2 weeks until week 19. Numbers in columns reflect the species frequency among 10 identified isolates per sampling occasion. Species were identified by rDNA gene sequencing using primers 16Ss/16Sr.
1
Below detection level (100 cfu g−1 grain).