Table 5.
Yeast species isolated from fermented dried crimped barley (FD); fermented moist crimped barley (FM); fermented moist crimped barley storage-inoculated with Wickerhamomyces anomalus (FW); fermented moist crimped barley storage-inoculated with Wickerhamomyces anomalus and LAB starter culture; additional LAB starter culture inoculated at the beginning of fermentation (FWLAB).
| Treatment | FD | FM | FW | FWLAB | ||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Week | 0 | 2 | 4 | 6 | 8 | 10 | 12 | 0 | 2 | 4 | 6 | 8 | 10 | 12 | 0 | 2 | 4 | 6 | 8 | 10 | 12 | 0 | 2 | 4 | 6 | 8 | 10 | 12 |
| Debaryomyces hansenii | 1 | |||||||||||||||||||||||||||
| Kazachstania exigua | 4 | 9 | 6 | 10 | 8 | 10 | 1 | 8 | 10 | 9 | 10 | 10 | 3 | 7 | 10 | 10 | 7 | 8 | 10 | 10 | ||||||||
| Pichia fermentans | 2 | |||||||||||||||||||||||||||
| Pichia kudriavzevii | 1 | 9 | 3 | 1 | 1 | 9 | 6 | 2 | 3 | 10 | 2 | 1 | ||||||||||||||||
| Pichia membranifaciens | 6 | 1 | ||||||||||||||||||||||||||
| Saccharomyces castellii | 4 | 1 | ||||||||||||||||||||||||||
| Wickerhamomyces anomalus | 4 | 1 | 4 | 5 | 1 | 1 | 4 | 8 | 4 | 3 | 8 | 9 | 3 | 2 | ||||||||||||||
Samples were taken after 1 week of undisturbed fermentation and then consecutively every 2 weeks until week 12 (equivalent to week 19 of storage trial). Numbers in columns reflect the species frequency among 10 identified isolates per sampling occasion. Species were identified by D1D2 rDNA gene sequencing using primers NL1/NL4.