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(A) Time-dependent expression of C. sinensis recombinant 7-kDa protein. Aliquots taken from the yeast culture medium at the indicated time intervals (in days) were analyzed by SDS-PAGE. Mr, molecular size in kilodaltons; lane 0, uninduced yeast supernatant. Lanes 1 to 6, yeast supernatant 1 to 6 days after induction with methanol. (B) Purification of recombinant protein by molecular sieve chromatography. Lanes 2 and 4, 2 and 4 μg of the purified proteins, respectively.
