Figure 2.

Generation of the recombinant Mgp-lacZ knock-in mouse. Top, an schematic representation of the cross between the Mgp-Cre Knock-in mouse and the Cre-mediated reporter R26R-lacZ to generate the Mgp-lacZ mouse. Bottom, genotyping of the genomic DNA from the Mgp-lacZ and control mice: left; primer pairs 1, 4 (Fig. 1) 6, and 7 (Jackson laboratories); right, primer pairs 8 and 9 to identify the rd8 mutation (primer sequences in methods).²⁹ All amplimers match the expected sizes. Mgp-lacZ mouse does not harbor the rd8 mutation in the Crb1 gene. lacZ, β-galactosidase.