TABLE 2.
Expression profile of unstimulated porcine PBMCs cultivated in vitro for 3, 12, 24, and 48 h
| Gene productb | Relative expression (AU) ata:
|
|||
|---|---|---|---|---|
| 3 h | 12 h | 24 h | 48 h | |
| ICAM | 81.9 ± 10.2 | 28.0 ± 1.3 | 5.3 ± 1.6 | 5.7 ± 1.2 |
| MCP-1 | 2.5 ± 0.5 | 10.4 ± 2.0 | 11.9 ± 2.6 | 27.8 ± 2.9 |
| NCP-1 | 3.0 ± 0.2 | 4.7 ± 0.2 | 3.3 ± 1.0 | 2.9 ± 0.8 |
| IL-6Rα | 2.9 ± 1.1 | 2.4 ± 1.2 | 2.3 ± 2.2 | 1.0 ± 0.1 |
| IL-2Rα | 2.3 ± 0.9 | 1.4 ± 0.2 | 1.2 ± 0.2 | 1.1 ± 0.1 |
| IL-4 | 2.1 ± 0.5 | 1.4 ± 0.1 | 1.3 ± 0.1 | 1.2 ± 0.2 |
| IL-8 | 2.6 ± 0.8 | 1.1 ± 0.3 | 1.3 ± 0.6 | 0.9 ± 0.4 |
| GST-O1 | 2.3 ± 0.7 | 1.9 ± 0.2 | 1.8 ± 0.5 | 1.7 ± 0.0 |
| MCP-2 | 1.3 ± 0.0 | 3.2 ± 0.5 | 5.8 ± 3.8 | 7.6 ± 0.3 |
| NRAMP | 1.5 ± 0.0 | 2.3 ± 0.1 | 2.9 ± 0.1 | 2.1 ± 0.2 |
| VCAM | 1.8 ± 0.4 | 1.7 ± 0.0 | 1.4 ± 0.1 | 1.9 ± 0.3 |
| CD1 | 1.7 ± 0.1 | 1.5 ± 0.1 | 1.3 ± 0.2 | 1.2 ± 0.1 |
| CD59 | 1.8 ± 0.0 | 1.5 ± 0.3 | 1.3 ± 0.2 | 1.0 ± 0.3 |
| CD80 | 1.4 ± 0.0 | 1.3 ± 0.1 | 1.1 ± 0.0 | 1.0 ± 0.1 |
| CD95 | 1.0 ± 0.0 | 1.1 ± 0.0 | 1.1 ± 0.2 | 0.9 ± 0.1 |
| AMCF-1 | 1.5 ± 0.1 | 1.1 ± 0.3 | 1.2 ± 0.6 | 0.5 ± 0.4 |
| BRAK | 1.4 ± 0.2 | 1.1 ± 0.4 | 1.0 ± 0.1 | 0.9 ± 0.1 |
| MIP-3β | 1.5 ± 0.1 | 1.3 ± 0.2 | 1.0 ± 0.2 | 0.9 ± 0.2 |
| RANTES | 1.1 ± 0.1 | 1.4 ± 0.2 | 1.4 ± 0.1 | 0.6 ± 0.1 |
| SDF-1β | 2.0 ± 0.3 | 1.4 ± 0.1 | 1.1 ± 0.1 | 1.1 ± 0.0 |
| SLC | 1.7 ± 0.2 | 1.5 ± 0.4 | 1.3 ± 0.0 | 1.1 ± 0.2 |
| TECK | 1.8 ± 0.1 | 1.6 ± 0.3 | 1.1 ± 0.2 | 1.1 ± 0.2 |
| COX1 | 1.3 ± 0.1 | 1.0 ± 0.3 | 0.9 ± 0.2 | 0.8 ± 0.1 |
| GM-CSF | 1.8 ± 0.6 | 1.7 ± 0.6 | 1.5 ± 0.7 | 0.9 ± 0.2 |
| IFN-α | 1.3 ± 0.2 | 1.2 ± 0.1 | 0.9 ± 0.1 | 1.2 ± 0.1 |
| IFN-γ | 1.9 ± 0.1 | 1.3 ± 0.1 | 1.2 ± 0.2 | 1.0 ± 0.0 |
| IL-2 | 1.5 ± 0.1 | 1.3 ± 0.3 | 1.0 ± 0.2 | 0.9 ± 0.1 |
| IL-5 | 1.7 ± 0.1 | 1.5 ± 0.1 | 1.3 ± 0.1 | 1.2 ± 0.3 |
| IL-6 | 2.0 ± 0.2 | 1.5 ± 0.3 | 1.1 ± 0.3 | 1.1 ± 0.2 |
| IL-7 | 1.4 ± 0.1 | 1.1 ± 0.2 | 1.0 ± 0.2 | 0.9 ± 0.2 |
| IL-10 | 1.7 ± 0.8 | 1.2 ± 0.3 | 0.8 ± 0.2 | 0.7 ± 0.2 |
| IL-12p35 | 1.2 ± 0.2 | 1.2 ± 0.2 | 1.1 ± 0.1 | 1.0 ± 0.2 |
| IL-12p40 | 1.5 ± 0.2 | 1.3 ± 0.0 | 1.1 ± 0.1 | 1.1 ± 0.1 |
| IL-13 | 1.7 ± 0.2 | 1.5 ± 0.1 | 1.2 ± 0.0 | 1.4 ± 0.1 |
| IL-15 | 1.3 ± 0.1 | 1.2 ± 0.3 | 1.0 ± 0.3 | 1.0 ± 0.3 |
| IL-16 | 1.5 ± 0.6 | 1.3 ± 0.1 | 1.2 ± 0.1 | 1.2 ± 0.2 |
| IL-18 | 1.8 ± 0.3 | 1.5 ± 0.2 | 1.2 ± 0.3 | 1.0 ± 0.3 |
| TGF-β2 | 1.8 ± 0.6 | 1.2 ± 0.2 | 1.1 ± 0.0 | 1.2 ± 0.2 |
| TNF-α | 1.0 ± 0.1 | 0.8 ± 0.2 | 0.7 ± 0.2 | 0.7 ± 0.1 |
| GST-A3 | 1.4 ± 0.2 | 1.3 ± 0.3 | 0.9 ± 0.3 | 0.9 ± 0.2 |
| GST-M1 | 1.8 ± 0.6 | 1.6 ± 0.3 | 1.5 ± 0.1 | 1.5 ± 0.3 |
| GST-P1 | 0.8 ± 0.1 | 1.5 ± 0.2 | 1.4 ± 0.1 | 1.8 ± 0.2 |
| GST-T2 | 1.5 ± 0.2 | 1.3 ± 0.4 | 1.1 ± 0.3 | 0.9 ± 0.3 |
| IL-8R1 | 1.4 ± 0.2 | 1.1 ± 0.2 | 0.8 ± 0.2 | 0.7 ± 0.1 |
| TGF-β 3R | 1.9 ± 0.6 | 1.6 ± 0.1 | 1.3 ± 0.0 | 1.2 ± 0.1 |
| TNF-R | 1.3 ± 0.0 | 1.6 ± 0.1 | 1.4 ± 0.1 | 1.4 ± 0.1 |
| NKG2D | 1.5 ± 0.2 | 1.1 ± 0.3 | 0.9 ± 0.2 | 0.6 ± 0.1 |
| DAP-12 | 1.3 ± 0.2 | 1.7 ± 0.1 | 1.6 ± 0.1 | 1.6 ± 0.2 |
| CTLA4 | 2.0 ± 0.2 | 1.5 ± 0.2 | 1.1 ± 0.1 | 1.0 ± 0.2 |
| ICE | 1.9 ± 0.2 | 1.5 ± 0.3 | 1.1 ± 0.1 | 1.1 ± 0.3 |
| iNOS | 1.4 ± 0.1 | 1.1 ± 0.2 | 0.9 ± 0.2 | 0.9 ± 0.1 |
| IL-1α | 1.1 ± 0.1 | 0.9 ± 0.3 | 0.7 ± 0.3 | 0.4 ± 0.2 |
| IL-1β | 1.3 ± 0.1 | 0.9 ± 0.5 | 0.9 ± 0.4 | 0.3 ± 0.2 |
| CD40 | 0.7 ± 0.1 | 0.5 ± 0.1 | 0.4 ± 0.1 | 0.4 ± 0.1 |
| MHC class II | 0.6 ± 0.1 | 0.3 ± 0.0 | 0.3 ± 0.0 | 0.2 ± 0.0 |
| TGF-β1 | 0.6 ± 0.1 | 0.3 ± 0.0 | 0.3 ± 0.0 | 0.3 ± 0.1 |
a
Data are expressed as the means ± standard errors of the means of values obtained from three animals relative to those at time zero. Boldface values (mean, >2.0) indicate gene overexpression, and underlined values (mean, <0.5) indicate gene underexpression. AU, arbitrary units.
b
Abbreviation explanations can be found in the gene product description column of Table 1.